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P. R. Thorat ¹, R. V. Kale ¹

Affiliations
1 Department of Microbiology, Shri Shivaji Mahavidyalaya, Barshi-413 411, Maharashtra, IN

Abstract

Degradation of the textile dyes viz., Yellow 4G and Orange 2R was carried out using acclimatized soil bacterial isolates. The microbial flora from soil was acclimatized to the dyes and six promising organisms were isolated, which could decolorize 1000 μg/mL of dyes to more than 91.00 % in nutrient medium in less than 24 hours. The percent decolorization of the dyes was determined by spectrophotometry. The six isolates reduced COD more than 75%. The degradation products formed after degradation were analysed by GC-MS technique and it was found that these cultures together degraded Yellow 4G and Orange 2R to the products having molecular weights 149, 65, 60, 57, 43, 41 and 271, 159, 145, 107, 102, 91, 81, 61, 55, 43 and 41 respectively.

Keywords

Textile Dyes, Degradation of Dyes, Decolourization, Aerobic Bacteria.

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R. V. Kale ¹, M. M. Sabale ¹, P. R. Thorat ¹

Affiliations
1 P.G. Dept. of Microbiology, Shri Shivaji Mahavidyalaya, Barshi-413 411, Maharashtra, IN

Abstract

Degradation and decolorization of azo dye Golden Yellow HE2R was carried out by soil bacterial isolates. Soil samples from various places were collected and acclimatized for decolorization of azo dye Golden Yellow HE2R. The acclimatized culture GY-B could decolorize 90% of the dye with 10,000 mg/L concentration. Eight promising isolates were able to decolorize the dye solution more than 83.43% averagely in less than 24 hours. Percent decolorization was studied by spectrophotometry. Hyperinteractions between carbon source like 1% glucose, 1% starch and 1% yeast extract and bacteria to decolorize the dye was investigated and modelled. In percent COD reduction studies, it was observed that more than 75% COD was reduced after decolorization process. The decolorization of the dye in half strength nutrient broth was also studied. The products formed after the degradation process were analysed by GC-MS technique.

Keywords

Degradation, Decolorization, Azo Dye, Carbon Source, COD Reduction.

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U. N. Jadhav ¹, P. R. Thorat ², A. M. Deshmukh ³

Affiliations
1 Deptt. of Microbiology, Baba Naik Mahavidyalaya, Kokrud, Dist. Sangli, Maharashtra, IN
2 P.G. Deptt. of Microbiology, Shri Shivaji Mahavidyalaya, Barshi, Dist. Solapur, Maharashtra, IN
3 Department of Microbiology, Dr. Babasaheb Ambedkar Marathwada University, Osmanabad, Maharashtra, IN

Abstract

The potential for Micromonospora spp. (Non Streptomyces Actinomycete) isolated from Vasant Sagar Dam, located in Sangli district of Maharashtra, was studied to decolorize dye wastewater from textile industry (Rem-Red and Rem-blue). Micromonospora spp. successfully decoulorised the textile wastewater containing two dyes, Rem-red and Rem-blue. Decolourisation studies were carried by inoculating intact cells and cellular extracts of Micromonospora spp. The maximum decolourisation was observed at pH 7.0 at five days incubation period at room temperature.

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